1׃ԣеĚIpY(ji)(gu)ɢ_γɟoҎ(gu)t朽Y(ji)(gu)^׃Ժጢʧȥ䲿ֻȫԡ׃Բ漰IĔһY(ji)(gu)Aֲ׃
2DNA׃Ժһϵ|(zh)ҲS֮l(f)׃
ߣɫЧ(yng)ȻB(ti)DNAȫ׃Ժt(y)W(xu)|W(wng)|ռգ260nmֵ,ɫЧ(yng)
TmֵSDNA׃ӣ260nmֵ̎uԓֵ_(d)ֵ50%r(sh)ĜضȷQĽ朜ضȻڽض“Tm”ʾ
TmֵӰأGCGCԽTmֵԽԽTmֵҲԽ
1W(wng)עԴt(y)W(xu)W(wng)Ʒ(qun)t(y)W(xu)W(wng)Уδ(jng)W(wng)ڙ(qun)D(zhun)d朽ӡD(zhun)Nʽʹѽ(jng)W(wng)ڙ(qun)(yng)ڙ(qun)(ni)ʹұעԴt(y)W(xu)W(wng)`ߣW(wng)䷨؟(z)
2W(wng)YϞW(wng)ѼD(zhun)dM(bio)ߺͳ̎ڱW(wng)dƷ漰(qun)Ȇ}ՈcW(wng)վ(lin)ϵW(wng)վˌ(sh)_J(rn)(hu)M̎
W(wng)D(zhun)d֮ƷζJ(rn)ͬԓƷ^c(din)挍(sh)ԡýwW(wng)վ(g)D(zhun)dʹՈc(qun)(lin)ϵؓ(f)؟(z)
3W(wng)վgӭeOͶ
4(lin)ϵʽ
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